Key result
Millimolar absorptivities of oxyhemoglobin and cyanmethemoglobin proved to be independent of total hemoglobin concentration, confirming earlier literature values.
Population
Human hemoglobin samples (in vitro)
Design
Preclinical
Authors
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Supports spectrophotometric hemoglobin assays in labs; confirms prior values in animal models but leaves open human translation.
The study confirms that the absorption spectra of human hemoglobin are independent of total hemoglobin concentration, validating widely used spectrophotometric methods for blood analysis.
Zijlstra et al. (1983) studied this question. Measurement of millimolar absorptivities of oxyhemoglobin and cyanmethemoglobin was evaluated on Millimolar absorptivities of oxyhemoglobin and cyanmethemoglobin at various wavelengths over a wide range of concentrations. Millimolar absorptivities of oxyhemoglobin and cyanmethemoglobin proved to be independent of total hemoglobin concentration, confirming earlier literature values.
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