The time at which ovulation occurs following intravenous injection of appropriate hormone preparations into pretreated and normally laying (non-pretreated) hens has been determined. The interval in pretreated hens averaging more than 2 ovulations per hen at time of autopsy was 6.8 hours, range 6.1-7.2 hours. Ninety percent of all normally laying hens (69) ovulated from 6.5 to 8.5 hours following ovulatory injection, the remaining 10% of injected hens requiring up to 9.6 hours for ovulation. The non-pretreated hens were injected with 4 different hormone preparations administered at differing dosage levels and at times calculated to effect ovulation of follicles at varying degrees of prematurity. The minimal ovulatory interval recorded for pretreated hens was 6.1 hours, for non-pretreated hens, 6.5 hours.
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Fraps et al. (1942) studied this question.