What question did this study set out to answer?

To identify genetic interactors of the microtubule-associated protein Stu1 and elucidate the molecular function of Ldb18 within the dynactin complex.

February 1, 2008GeneticsOpen Access

The Saccharomyces cerevisiae Homolog of p24 Is Essential for Maintaining the Association of p150Glued With the Dynactin Complex

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To identify genetic interactors of the microtubule-associated protein Stu1 and elucidate the molecular function of Ldb18 within the dynactin complex.
Conducted a genome-wide synthetic lethality screen in Saccharomyces cerevisiae using the temperature-sensitive stu1-5 allele.
Assessed protein-protein interactions and complex integrity using yeast two-hybrid screens, co-immunoprecipitation, and 20 S sucrose gradient sedimentation.
Identified ldb18Δ as synthetically lethal with stu1-5 and Kar9 pathway mutations, reproducing spindle orientation defects typical of dynein pathway loss.
Demonstrated that Ldb18 functions as a p24 homolog required for linking Nip100 (p150Glued) to Jnm1 (dynamitin), while leaving the Jnm1-Arp1 interaction intact.

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Implication

Genetic and biochemical analysis reveals that Ldb18 connects p150Glued to dynactin in yeast, indicating its essential role in dynein-dependent spindle orientation.

Cite This Study

Amaro et al. (2008) studied this question.

synapsesocial.com/papers/6aa038b8a2fa39ae1c348cf5 https://doi.org/10.1534/genetics.107.079103

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Implication

Genetic and biochemical analysis reveals that Ldb18 connects p150Glued to dynactin in yeast, indicating its essential role in dynein-dependent spindle orientation.

The Saccharomyces cerevisiae Homolog of p24 Is Essential for Maintaining the Association of p150Glued With the Dynactin Complex

Key Points

Discussion

Implication

Cite This Study

The Saccharomyces cerevisiae Homolog of p24 Is Essential for Maintaining the Association of p150Glued With the Dynactin Complex

Key Points

Discussion

Implication

Cite This Study