18F‑fluorodeoxyglucose positron emission tomography for the detection of inflammatory lesions of the arterial vessel walls in Wistar rats

Q: What is the clinical evidence from this study?

Population: Inflammatory lesions of the arterial vessel walls (atherosclerosis model) (n=20). Intervention: High-fat and high-salt diet vs. Normal diet. Primary outcome: Standardized uptake values (SUVs) of 18F-FDG in the iliac artery at 24 weeks (p=<0.05).

Q: What question did this study set out to answer?

To evaluate the efficacy of 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) in detecting arterial wall inflammation induced by a high-fat and high-salt diet in Wistar rats.

Shiwei Shen; Hongwei Li; Ge Song; Hongbo Huang; Hui Zhang; Feng Li; Yinbo Feng; Ling Wang; Xiaofeng Weng; Yun Lu; Zhenhai Shen

doi:10.3892/etm.2021.9801

February 19, 2021Experimental and Therapeutic MedicineOpen Access

18F‑fluorodeoxyglucose positron emission tomography for the detection of inflammatory lesions of the arterial vessel walls in Wistar rats

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Key result

A high-fat and high-salt diet significantly increased 18F-FDG uptake in the iliac artery of Wistar rats at 24 weeks compared to a normal diet (SUV 1.96 vs 1.12, P<0.05).

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Why the study?

The study aimed to evaluate 18F-FDG PET for detecting high-fat and high-salt diet-induced inflammatory lesions of arterial vessel walls in Wistar rats.

Does 18F-FDG PET detect inflammatory lesions in the arterial vessel walls of Wistar rats on a high-fat and high-salt diet?

Key Points

To evaluate the efficacy of 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) in detecting arterial wall inflammation induced by a high-fat and high-salt diet in Wistar rats.
Randomly assigned 20 healthy 8-week-old male Wistar rats to a high-fat diet or normal diet group.
Administered 18F-FDG at 1 mCi/kg via tail vein after 12-hour fasting at 16 and 24 weeks (n=5 per group per time point), followed by 10-minute micro-PET imaging under 2% isoflurane anesthesia.
Performed immunohistochemical staining of arterial vessels to quantify CD68-positive macrophage infiltration.
Standardized uptake values (SUVs) in the iliac artery were significantly higher in the high-fat diet group than the normal diet group at 16 weeks (1.53±0.08 vs. 1.04±0.03; P<0.05) and 24 weeks (1.96±0.17 vs. 1.12±0.07; P<0.05).
Abdominal aorta SUVs were significantly elevated in the high-fat diet group compared with the normal diet group at 16 weeks (1.35±0.08 vs. 1.02±0.02; P<0.05) and 24 weeks (1.54±0.09 vs. 1.04±0.02; P<0.05), with high-fat uptake increasing significantly between 16 and 24 weeks (P<0.05).
CD68-positive macrophage density in the high-fat diet group at 24 weeks (6.94±2.02%) was significantly higher than in the high-fat group at 16 weeks (4.70±2.02%) and the normal diet group at 24 weeks (3.20±1.80%; P<0.05).

Structured PICO

Does 18F-FDG PET detect inflammatory lesions in the arterial vessel walls of Wistar rats on a high-fat and high-salt diet?

P

Population

20 healthy, 8-week-old, male Wistar rats fed a high-fat and high-salt diet or normal diet for up to 24 weeks to model inflammatory lesions of arterial vessel walls.

I

Intervention

High-fat and high-salt diet (49% fat, 21% protein, 30% carbohydrate, 2% salt) for 16-24 weeks, followed by 18F-FDG PET imaging

C

Comparator

Normal diet (10% fat, 22% protein, 68% carbohydrate, 0.5% salt) for 16-24 weeks

O

Outcome

Standardized uptake values (SUVs) of 18F-FDG in the iliac artery and abdominal aortasurrogate

Main Result

Absolute Event Rate: 1.96% vs 1.12%

p-value: p=<0.05

18F-FDG PET can noninvasively detect and monitor early inflammatory lesions and macrophage accumulation in the arterial walls of a rat model of atherosclerosis.

Limitations

The rat model of the early inflammatory stage of atherosclerosis lacks evidence of inflammatory markers, such as leukocytes, high sensitivity C-reactive protein, myeloperoxidase, LI-6, and monocyte chemoattractant protein-1.
The results may be more meaningful if ultrasound was used to measure the thickness of the abdominal aorta and the feeding time was prolonged.
Rats have no gallbladder, making it difficult to induce a rise in cholesterol and produce typical atherosclerotic plaques with diet alone

Implication

18F-FDG PET detects diet-induced arterial inflammation in rats; hypothesis-generating for human vascular imaging, pending clinical validation.

Abstract

The present study aimed to evaluate the use of 18F‑fluorodeoxyglucose (18F‑FDG) positron emission tomography (PET) for detection of high‑fat and high‑salt diet‑induced inflammatory lesions of the arterial vessel walls in Wistar rats. A total of 20 healthy, 8‑week‑old, male Wistar rats were randomly assigned to the high‑fat diet group and the normal diet group. After 16 and 24 weeks of feeding, Wistar rats in the normal diet group and the high‑fat diet group (five rats in each group) were injected with 18F‑FDG through the tail vein at a dose of 1 mCi/kg after fasting for 12 h. After 1 h, the rats were anesthetized with 2% isoflurane, followed by micro‑PET imaging with a 10‑min image capture duration and immunohistochemical staining. The standardized uptake values (SUVs) of 18F‑FDG were significantly higher in the iliac artery in the high‑fat diet group compared with those in the normal diet group at 16 weeks (1.53±0.08 vs. 1.04±0.03; P<0.05) and at 24 weeks (1.96±0.17 vs. 1.12±0.07; P<0.05). The SUVs of 18F‑FDG were also significantly greater in the abdominal aorta in the high‑fat diet group compared with those in the normal diet group at 16 weeks (1.35±0.08 vs. 1.02±0.02; P<0.05) and at 24 weeks (1.54±0.09 vs. 1.04±0.02; P<0.05). In addition, the SUVs of 18F‑FDG in the iliac artery and abdominal aorta were significantly higher at 24 weeks compared with those at 16 weeks in the high‑fat diet group (P<0.05). As determined by immunohistochemistry, the percentage of CD68‑positive cells in the total number of cells per unit area in each group was 3.20±1.80% in the 24‑week normal diet group, 4.70±2.02% in the 16‑week high‑fat diet group and 6.94±2.02% in the 24‑week high‑fat diet group; the percentage of CD68‑positive cells in the high‑fat diet group at 24 weeks was significantly higher than that in the high‑fat diet group at 16 weeks and in the normal diet group at 24 weeks (P<0.05). In conclusion, 18F‑FDG PET is a noninvasive imaging tool that can continuously monitor inflammatory lesions of the arterial vessel walls in Wistar rats. Further improvement of the Wistar rat atherosclerosis model may provide data to support the early assessment of and intervention in atherosclerosis.

Discussion

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Cite This Study

Shen et al. (2021) studied Inflammatory lesions of the arterial vessel walls (atherosclerosis model) (n=20). High-fat and high-salt diet vs. Normal diet was evaluated on Standardized uptake values (SUVs) of 18F-FDG in the iliac artery at 24 weeks (p=<0.05). A high-fat and high-salt diet significantly increased 18F-FDG uptake in the iliac artery of Wistar rats at 24 weeks compared to a normal diet (SUV 1.96 vs 1.12, P<0.05).

synapsesocial.com/papers/6aa03a16bdf28614dc2d26ec https://doi.org/10.3892/etm.2021.9801

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18F‑fluorodeoxyglucose positron emission tomography for the detection of inflammatory lesions of the arterial vessel walls in Wistar rats

Figures

Key Points

Structured PICO

Main Result

Limitations

Implication

Abstract

Discussion

Cite This Study

18F‑fluorodeoxyglucose positron emission tomography for the detection of inflammatory lesions of the arterial vessel walls in Wistar rats

Figures

Key Points

Structured PICO

Main Result

Limitations

Implication

Abstract

Discussion

Cite This Study

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