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August 30, 2013PLoS ONEOpen Access

CYP2J2 Overexpression Protects against Arrhythmia Susceptibility in Cardiac Hypertrophy

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Key result

Cardiomyocyte-specific overexpression of CYP2J2 completely prevented ventricular tachyarrhythmia inducibility (0% vs 47%) and reduced mortality (6% vs 42%) in mice subjected to chronic pressure overload.

Why the study?

Does CYP2J2 overexpression reduce arrhythmia susceptibility in mouse models of cardiac hypertrophy?

Population

Male transgenic mice with cardiomyocyte-specific overexpression of the human epoxygenase CYP2J2 and wildtype…

Comparison

Cardiomyocyte-specific overexpression of the… vs Wildtype littermates (WT)

Design

Preclinical

Follow-up

Up to 8 weeks (TAC) or 14 days (ISO)

Authors

CWChristina WestphalBSBastian SpallekAKAnne Konkel

Discussion

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Member takes

Overview

Hypothesis-generating for CYP2J2 in hypertrophy-related arrhythmias; leaves open translation to human disease.

Structured PICO

Does CYP2J2 overexpression reduce arrhythmia susceptibility in mouse models of cardiac hypertrophy?

P
Population
Male transgenic mice with cardiomyocyte-specific overexpression of CYP2J2 and wildtype littermates subjected to chronic pressure overload or isoproterenol infusion to induce cardiac hypertrophy.
I
Intervention
Cardiomyocyte-specific overexpression of the human epoxygenase CYP2J2 (CYP2J2-TG)
C
Comparator
Wildtype littermates (WT)
O
Outcome
Mortality, ventricular tachyarrhythmia inducibility, and atrial fibrillation vulnerabilitysurrogate

Main Result

Absolute Event Rate: 0% vs 47%

p-value: p=<0.05

Enhanced cardiac EET biosynthesis via CYP2J2 overexpression protects against electrical remodeling and arrhythmia susceptibility in mouse models of maladaptive cardiac hypertrophy.

Limitations

  • Animal model findings may not directly translate to human clinical outcomes
  • Further studies are necessary to understand the actual molecular mechanisms linking EET-mediated mitochondrial K(ATP) channel activation to protection against Cx43 redistribution and arrhythmia

Cite This Study

Westphal et al. (2013) studied Cardiac hypertrophy. CYP2J2 overexpression vs. Wildtype (WT) littermates was evaluated on Ventricular tachyarrhythmia inducibility 4 weeks after transverse aortic constriction (TAC) (p=<0.05). Cardiomyocyte-specific overexpression of CYP2J2 completely prevented ventricular tachyarrhythmia inducibility (0% vs 47%) and reduced mortality (6% vs 42%) in mice subjected to chronic pressure overload.

synapsesocial.com/papers/6aa03e4507dbf0d1b4e2df75https://doi.org/10.1371/journal.pone.0073490
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Cardiomyocyte-specific expression of CYP2J2 prevents development of cardiac remodelling induced by angiotensin II2015 · 67 citations
  2. 2Enhanced Postischemic Functional Recovery in CYP2J2 Transgenic Hearts Involves Mitochondrial ATP-Sensitive K + Channels and p42/p44 MAPK Pathway2004 · 272 citations
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