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An improved method for the electron microscopic study of intact tissue culture cells is based on the use of carbon-collodion coated stainless steel grids upon which the cells are grown. Cell preparations are fixed with buffered osmium and metal shadowed. This technic has been applied to the study of 5 cell lines including mouse fibroblasts strain “L” (Earle), HeLa (Gey), liver epithelial (Chang), chicken fibroblasts and chicken monocytes. Distinctive surface features of these cell lines are described.
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Overman et al. (1961) studied this question.
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