Nucleoside‐Diphosphate Derivatives of 2‐Deoxy‐ d ‐glucose in Animal Cells

After administration of 14C‐labelled 2‐deoxy‐d‐glucose or 3H‐labelled nucleosides to cells in tissue culture the following activated intermediates of the deoxysugar have been isolated and characterized: the 6‐phosphate derivative, the 1‐phosphate derivative, the 1,6‐bisphosphate derivative, UDP‐2‐deoxy‐d‐glucose, GDP‐2‐deoxy‐d‐glucose and 2‐deoxy‐d‐gluconic acid 6‐phosphate. After extraction with acid and organic solvents, 2‐deoxy‐d‐glucose could be recovered by acid hydrolysis from the cells. Labelled 2‐deoxy‐d‐galactose was not found either bound to UDP or to the cells, indicating that no significant epimerisation of 2‐deoxy‐d‐glucose at the UDP level occurred in these cells.