Key result
The ratio of RNA genomes to infectious poliovirus 1 increased from 10(0) to 10(3) after 600 days at 4 degrees C, and to 10(4) after 200 days at 22 degrees C.
Population
Three different enteroviruses in artificial ground and surface waters
Design
Preclinical
Follow-up
up to 600 days
Authors
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May overestimate infectious virus by molecular detection in stored water; leaves open validation of viability assays for environmental monitoring.
When using molecular detection for virus enumeration in water, the fraction of infectious virus decays with time, especially at higher temperatures.
Husman et al. (2008) studied this question. Different controlled pH, temperature, and salt conditions was evaluated on Ratio of RNA genomes to infectious virus over time. The ratio of RNA genomes to infectious poliovirus 1 increased from 10(0) to 10(3) after 600 days at 4 degrees C, and to 10(4) after 200 days at 22 degrees C.
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