Key result
Whole blood platelet aggregometry demonstrated good agreement with the platelet-rich plasma reference method and moderate agreement with the Multiplate analyzer for assessing aspirin responsiveness.
Why the study?
Does whole blood platelet aggregometry agree with platelet rich plasma aggregometry in assessing platelet responsiveness to aspirin?
Observational (n=65)
Does whole blood platelet aggregometry agree with platelet rich plasma aggregometry in assessing platelet responsiveness to aspirin?
Whole blood platelet aggregation demonstrates acceptable agreement with the reference platelet rich plasma method for assessing aspirin responsiveness.
Acceptable agreement supports whole blood aggregometry as alternative; leaves open outcome validation before practice change.
Assessment on platelet responsiveness to aspirin may be required in selected clinical conditions. So far, no standardization in laboratory practices for aspirin assessment using whole blood (WB) platelet aggregation based test. A study for method validation was performed to investigate the aspirin effects on platelets by comparing with a reference method. Forty patients taking aspirin were analyzed using WB by conventional platelet aggregometer (Chrono-Log Model 500CA/560CA). Among these patients, nine of them had their platelet rich plasma (PRP) tested by the same analyzer (reference method). Another WB specimens from 25 patients were tested on both Chrono-Log and Multiplate® (Dynabyte GmbH), which is a newer generation platelet function analyzer. There were good and moderate agreements between WB on the Chronolog analyzer vs the reference method and WB on Chronolog vs WB on Multiplate® analyzers respectively. There are agreements between PRP and WB aggregation (WBA) methods in detecting aspirin effects on platelets. It is recommended that the test validation for the assessment of platelet responsiveness to aspirin is interpreted and correlated with the reference method preferably PRP.
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Abdullah et al. (2013) conducted an observational in Patients taking aspirin (n=65). Whole blood platelet aggregometry vs. Platelet rich plasma aggregometry and Multiplate analyzer was evaluated on Agreement between whole blood platelet aggregometry and reference methods. Whole blood platelet aggregometry demonstrated good agreement with the platelet-rich plasma reference method and moderate agreement with the Multiplate analyzer for assessing aspirin responsiveness.
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