Three experiments were conducted in which old hens, young hens and old hens producing a high percentage of shell-less and ultra-thin-shelled eggs were used. Birds in each category were divided into two groups. They were fed either a layer diet containing 3.00% calcium, 0.75% phosphorus and 2200 I.C.U. of vitamin D3 per kg. of feed or the same diet plus 1.1 mg. 25-OH-D3 per kg. of feed. The results of these experiments indicated that 25-OH-D3 had no significant effect on egg production, serum calcium, feed consumption, egg weight or specific gravity of eggs.
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Roland et al. (1976) studied this question.
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