Key result
Phosphomimetic mutations of phospholamban increased its oligomerization and altered the conformation of the PLB-SERCA regulatory complex in live AAV-293 cells.
Phosphomimetic mutations in phospholamban increase its oligomerization and alter the conformation of the PLB-SERCA regulatory complex without changing the inherent affinity of monomeric PLB for SERCA.
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Should not yet alter clinical management; leaves open PLB oligomerization effects on SERCA in human disease.
Hou et al. (2008) studied this question. Phosphomimetic mutations (S16E or S16E/T17E) of phospholamban was evaluated on PLB-PLB and PLB-SERCA interactions measured by FRET. Phosphomimetic mutations of phospholamban increased its oligomerization and altered the conformation of the PLB-SERCA regulatory complex in live AAV-293 cells.