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A high-performance liquid chromatographic method for the elucidation of the principal Citrus flavanone glycosides in biosynthesis and their respective dihydrochalcones has been developed. A C18 reverse phase column and an elution isocratic-gradient system is used with a mixture of water, acetonitrile and acetic acid. We describe the optimization process by studying the influence of the structural characteristics of these compounds on the quantitative chromatographic parameters: k′, w and R.
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Rı́o et al. (1994) studied this question.
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