Key result
Post-MI RNA sequencing in mice reveals a long-lived proinflammatory neutrophil subset reaching ~50% by day 4.
Why the study?
Neutrophils are thought to be short-lived first responders to tissue injury such as myocardial infarction, but little is known about their diversification or dynamics.
Population
>28 000 neutrophil transcriptomes from mice after MI or at steady-state
Comparison
Neutrophils isolated on days 1 to 4 after MI vs at steady-state
Design
Preclinical single-cell transcriptomic animal study
Follow-up
Days 1 to 4 after MI
Authors
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Single-cell profiling reveals neutrophil heterogeneity after MI in mice; hypothesis-generating for immune-targeted therapies in humans.
Single-cell transcriptomics reveals a dynamic, late-emerging proinflammatory SiglecF HI neutrophil subset in the infarcted murine heart that expresses longevity-associated genes.
Calcagno et al. (2021) studied Myocardial infarction. Myocardial infarction (LAD ligation) vs. Steady-state was evaluated on Neutrophil subsets and transcriptomes. Following myocardial infarction in mice, single-cell RNA sequencing identified a late-emerging, long-lived proinflammatory Ly6G+SiglecF(HI) neutrophil subset accounting for >50% of neutrophils by day 4.
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