MiRNA-10a is upregulated in NSCLC and may promote cancer by targeting PTEN

// Tao Yu 1,* , Lei Liu 1,* , Jing Li 1 , Mingxia Yan 1 , Hechun Lin 1 , Ying Liu 1 , Dandan Chu 1 , Hong Tu 1 , Aiqin Gu 2 and Ming Yao 1 1 State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China 2 Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China * Authors share co-first authorship Correspondence to: Ming Yao, email: // Aiqin Gu, email: // Keywords : NSCLC, metastasis, microRNA-10a, PTEN Received : April 15, 2015 Accepted : July 11, 2015 Published : July 22, 2015 Abstract MicroRNAs (miRNAs) are involved in human cancer including non-small cell lung cancer (NSCLC). In this study, we compared miRNA expression microarray of SPC-A-1sci (high metastatic) and SPC-A-1 (weakly metastatic) cells. We found that miRNA-10a was up-regulated in NSCLC compared with corresponding normal tissues. High expression of miR-10a was associated with tumor node metastasis and lymph node metastasis. Furthermore, overexpression of miR-10a promoted NSCLC cell proliferation, migration and invasion in vitro . We found that PTEN was a direct target of miR-10a in NSCLC. Also miR-10a activated the PTEN/AKT/ERK pathway. We suggest that miR-10a contributes to NSCLC by targeting PTEN.