A major simplification of the methyl coenzyme M methylreductase system of Methanobacterium has been effected. The 500,000-dalton hydrogenase complex has been replaced by an NADPH-coenzyme F420 oxidoreductase. By use of this electron-generating reaction, the methylreductase was found to be localized in component C, an acidic protein fraction. In the presence of the oxidoreductase and the methylreductase, formation of methane under a nitrogen atmosphere was dependent upon the addition of NADPH, coenzyme F420, component B (a new cofactor of unknown structure), ATP, Mg2+, and methyl coenzyme M.
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Ellefson et al. (1980) studied this question.
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