Electrochemical sensing of antipyrine and its interaction with ds-DNA modified electrode.

The interaction of antipyrine (AnP) with the double-stranded deoxyribonucleic acid of salmon sperm on a electrochemical electrode (GCE) was carefully examined using voltammetric methods.The Ultra violet-visible absorption method was used to determine the interaction of AnP and double strand DNA in solution. AnP give a single anodic peak in buffer solutions of (pH 3.5 - 9.5).AnP is oxidized in a diffusion-controlled process from a phosphate buffer solution, pH 7.The variation given by AnP through its current signals was monitored in the existance of DNA by GCE using different voltammetric techniques. Diffusion coefficient of free AnP was 3.7×10-6 and 2.8×10-7 cm2s-1 for the AnP-DNA .The binding constant was calculated to be 1.5×105 and 1.1×105 M-1 . AnP binds to DNA primarily by electrostatic interaction with a contribution from intercalation. A detection pattern depends on pre-concentration and DPV detection at GCE modified with dsDNA was introduced to determine AnP in saliva matrices.