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October 20, 2025Diagnostics7 citationsOpen Access

Quantitative Ultrasound for Hepatic Steatosis: A Systematic Review Highlighting the Diagnostic Performance of Ultrasound-Derived Fat Fraction

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DKDimitrios KavvadasVRVasileios RafailidisALAris Liakos

Key Result

Ultrasound-Derived Fat Fraction demonstrated a strong pooled correlation (r = 0.85) with MRI-PDFF and high diagnostic accuracy for grading hepatic steatosis.

Study Design

Type

Systematic Review (n=862)

Structured PICO

Does Ultrasound-Derived Fat Fraction (UDFF) accurately diagnose and grade hepatic steatosis compared to MRI-PDFF in patients with MASLD?

P
Population
862 adult patients with suspected or confirmed metabolic-dysfunction-associated steatotic liver disease from 7 prospective studies evaluating the diagnostic performance of Ultrasound-Derived Fat Fraction.
E
Exposure
Ultrasound-Derived Fat Fraction (UDFF)
C
Comparator
MRI Proton Density Fat Fraction (MRI-PDFF) as reference standard, and other modalities (Controlled Attenuation Parameter, histology, other quantitative ultrasound techniques)
O
Outcome
Diagnostic performance (correlation, reproducibility, and diagnostic accuracy/AUC)surrogate

UDFF is a reliable and cost-effective quantitative ultrasound modality that correlates strongly with MRI-PDFF for grading hepatic steatosis, though standardized protocols are needed before broad routine adoption.

Main Result

Effect estimate: r = 0.85 (95% CI 0.81-0.89)

Limitations

  • Variability of studies in terms of patient populations, device settings, and measurement protocols
  • UDFF is proprietary to a single vendor, limiting generalizability to other quantitative ultrasound platforms
  • Initial search was restricted to PubMed and Scopus
  • Cut-off variability
  • Protocol sensitivity (measurements influenced by body position, probe pressure, and visceral fat)

Abstract

Background/Objectives: Metabolic-dysfunction-associated steatotic liver disease (MASLD) is a leading cause of chronic liver disease worldwide, requiring accurate and accessible diagnostic tools. Methods: A systematic review evaluated the diagnostic performance of Ultrasound-Derived Fat Fraction (UDFF), with a primary focus on prospective studies comparing UDFF to MRI Proton Density Fat Fraction (MRI-PDFF) as the reference standard and a secondary appraisal of its performance against other modalities. Additional parameters, such as technical feasibility, inter-observer agreement, and proposed thresholds, were summarized to support clinical applicability. Results: Seven prospective MRI-based studies (n = 862) demonstrated excellent correlation (average r = 0.848) and reproducibility (inter-observer intraclass correlation coefficient ICC = 0.978, intra-observer ICC = 0.980) of UDFF, with high diagnostic accuracy across steatosis grades (AUCs ≥ 0.89). Additional studies comparing UDFF with Controlled Attenuation Parameter (CAP), histology, and other quantitative ultrasound techniques (attenuation- or backscatter-based methods) confirmed high sensitivity and specificity, particularly for advanced steatosis, and emphasized the potential of UDFF as a comprehensive quantitative biomarker. Proposed UDFF cut-offs for mild, moderate, and severe steatosis ranged from 5% to 23%, demonstrating high sensitivity and specificity. Factors like body position, probe pressure, and visceral fat influenced measurements, underscoring the need for standardized protocols. Conclusions: UDFF seems to offer a reliable and cost-effective quantitative ultrasound modality. So far, it correlates strongly with MRI-PDFF and accurately grades steatosis, especially for S2–S3. Given cut-off variability and protocol sensitivity, broad routine adoption may be premature. Therefore, we recommend further studies focusing on standardized acquisition and cut-off calibration to MRI-PDFF.

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Cite This Study

Kavvadas et al. (2025) conducted a systematic review in Hepatic steatosis (n=862). Ultrasound-Derived Fat Fraction (UDFF) vs. MRI Proton Density Fat Fraction (MRI-PDFF) was evaluated on Correlation between UDFF and MRI-PDFF (r = 0.85, 95% CI 0.81-0.89). Ultrasound-Derived Fat Fraction demonstrated a strong pooled correlation (r = 0.85) with MRI-PDFF and high diagnostic accuracy for grading hepatic steatosis.

synapsesocial.com/papers/6aa103bc25b16df10966768dhttps://doi.org/10.3390/diagnostics15202640
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