The composition of metabolites of cyanobacteria cultures of the Astrakhan region has been studied by thin-layer chromatography, high-performance thin layer chromatography, and high-performance liquid chromatography. Thin layer chromatography analysis of the cyanobacteria metabolites has shown the best separation and quality of chromatographic zones in seven systems: chloroform, ethanol: acetic acid (1: 1), butanol, ethanol: water (1: 9), butanol: acetic acid: water (4: 1: 5), ethanol: water (9: 1), and butanol: acetic acid: water (4: 1: 5). The flavonoid quercetin has been detected in an aqueous–alcoholic extract by high -performance thin-layer chromatography. The high-performance liquid chromatography has allowed identification of aspartic, isomonic, lactic, and fumaric acids in the cyanobacteria metabolites. Antioxidant activity of aqueous and aqueous-alcoholic extracts of the Phormidium ramosum B.-Peters IPPAS B-2022 culture has been detected.
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Bataeva et al. (2018) studied this question.
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