1 Abstract: The present study was conducted on maize, with the aim to work out a suitable surface disinfecting procedure for seeds and to know the best concentration of 2,4-dichlorophenoxyacetic acid (2,4 -D) for callus induction in N6 medium using var. EV-2097. Better disinfection of seeds was achieved with 30 minutes Clorox treatment (86 %). Maximum callus induction (47 %) was recorded with 2 mg l followed by 3 mg l (18 %) and 1 mg l 2,4 -D (16 %). 1 1 1
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Naqvi et al. (2002) studied this question.
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