What question did this study set out to answer?

To identify and biochemically characterize the enzyme responsible for converting sorbitol to glucose in apple leaf tissue.

A sorbitol oxidase that converts sorbitol to glucose in apple leaf1

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To identify and biochemically characterize the enzyme responsible for converting sorbitol to glucose in apple leaf tissue.
Extracted and partially purified a novel oxidase enzyme from apple leaves.
Evaluated reaction stoichiometry, cofactor dependence (NAD/NADP), cation sensitivity, pH optimum, and substrate affinity.
The partially purified enzyme converted sorbitol to glucose via oxygen consumption (sorbitol + 1/2 O2 → glucose + H2O) independently of NAD or NADP.
Enzyme activity was optimal at pH 4.0, displayed a Km value of 100 mM for sorbitol, and exhibited weak dependency on divalent cations.

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Biochemical analysis reveals a cofactor-independent sorbitol oxidase in apple leaves, suggesting a key role in plant carbohydrate metabolism.

Shohei Yamaki (1980) studied this question.

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Biochemical analysis reveals a cofactor-independent sorbitol oxidase in apple leaves, suggesting a key role in plant carbohydrate metabolism.