Key result
A newly developed multiplex real-time PCR assay for ASFV demonstrated 98.4% in silico strain coverage, a limit of detection of 6 plasmid copies, and 100% specificity, outperforming the Zsak assay.
Why the study?
More ASFV whole genome sequences became available after the widely used Zsak assay was designed, prompting a new assay with broader coverage and an internal control.
Population
26 ASFV isolates with different country origins, ACTB sequences, and p72 sequences
Comparison
Newly developed multiplex real-time PCR assay vs Zsak assay
Design
In vitro and in silico assay development and validation study
Authors
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New p72 PCR may improve ASFV detection in updated strains; leaves open prospective validation before clinical adoption.
A newly developed multiplex real-time PCR assay for ASFV demonstrates high sensitivity, specificity, and broader strain coverage compared to the widely used Zsak assay.
Wang et al. (2020) studied African swine fever virus (ASFV). New ASFV p72 gene-based multiplex real-time PCR assay vs. Zsak assay was evaluated on ASFV strain/isolate coverage, sensitivity, and specificity. A newly developed multiplex real-time PCR assay for ASFV demonstrated 98.4% in silico strain coverage, a limit of detection of 6 plasmid copies, and 100% specificity, outperforming the Zsak assay.
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