What is the clinical evidence from this study?

Study design: Other. Population: Porcine viral infections. Intervention: Multiplexed real-time quantitative PCR (qPCR) assay. Primary outcome: Detection of porcine viruses.

January 10, 2013Journal of Clinical MicrobiologyOpen Access

Single-Tube Multiplexed Molecular Detection of Endemic Porcine Viruses in Combination with Background Screening for Transboundary Diseases

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Key result

Novel multiplex RT-qPCR enables highly sensitive parallel detection of porcine and transboundary viruses without interference.

Population

A large panel of different body fluids and tissues from pigs and other animal species

Design

Preclinical

Authors

KWKerstin WernikeFriedrich-Loeffler-Institut BHBernd HoffmannFriedrich-Loeffler-Institut MBMartin BeerUniversity of Bern

Discussion

Member takes

Implication

May enable efficient multiplex screening for swine viruses; leaves open prospective validation before routine diagnostic use.

Structured PICO

Population

A large panel of different body fluids and tissues from pigs and other animal species (reference samples and clinical specimens)

Intervention

Single-tube multiplexed real-time quantitative PCR (RT-qPCR) assay for simultaneous detection of PRRSV, PCV2, and four transboundary viruses

Outcome

Sensitivity and specificity of parallel detection of the different virusessurrogate

A novel multiplex RT-qPCR assay enables simultaneous detection of endemic porcine pathogens and exclusion diagnostics for major transboundary diseases, potentially improving syndromic surveillance in swine.

Cite This Study

Wernike et al. (2013) studied Porcine viral infections. Multiplexed real-time quantitative PCR (qPCR) assay was evaluated on Detection of porcine viruses. A novel multiplex RT-qPCR system enabled highly sensitive and specific parallel detection of endemic porcine viruses and transboundary diseases without interference from high viral loads.

synapsesocial.com/papers/6aa14f1980250ee14fed6b9b https://doi.org/10.1128/jcm.02947-12

View Full Paper

Ask AI

Bookmark

Single-Tube Multiplexed Molecular Detection of Endemic Porcine Viruses in Combination with Background Screening for Transboundary Diseases

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Single-Tube Multiplexed Molecular Detection of Endemic Porcine Viruses in Combination with Background Screening for Transboundary Diseases

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider