Ordered and disordered proteins as nanomaterial building blocks

Proteins possess a number of attractive properties that have contributed to their recent emergence as nanoscale building blocks for biomaterials and bioinspired materials. For instance, the amino acid sequence of a protein can be precisely controlled and manipulated via recombinant DNA technology, and proteins can be biosynthesized with very high purity and virtually perfect monodispersity. Most importantly, protein-based biomaterials offer the possibility of technologically harnessing the vast array of functions that these biopolymers serve in nature. In this review, we discuss recent progress in the field of protein-based biomaterials, with an overall theme of relating protein structure to material properties. We begin by discussing materials based on proteins that have well-defined three-dimensional structures, focusing specifically on elastin- and silk-like peptides. We then explore the newer field of materials based on intrinsically disordered proteins, using nucleoporin and neurofilament proteins as case studies. A key theme throughout the review is that specific environmental stimuli can trigger protein conformational changes, which in turn can alter macroscopic material properties and function.