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Experimental study demonstrates label-free birefringence and phase mapping in unstained live cardiac cells, highlighting a novel approach for non-invasive cellular analysis.
A novel application of quantitative phase imaging under linearly polarized light is introduced for studying unstained anisotropic live cells. The method is first validated as a technique for mapping the two-dimensional retardation distribution of a well-characterized optical fiber and is then applied to the characterization of unstained isolated cardiac cells. The experimental retardation measurements are in very good agreement with the established Brace-Köhler method, and additionally provide spatially resolved cell birefringence and phase data.
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Dragomir et al. (2007) studied this question.
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