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December 1, 1975Proceedings of the National Academy of SciencesOpen Access

Applications of natural-abundance nitrogen-15 nuclear magnetic resonance to large biochemically important molecules.

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To assess the feasibility and spectroscopic dynamics of natural-abundance nitrogen-15 nuclear magnetic resonance spectroscopy in large, biologically significant macromolecules.
Applied newly developed instrumentation to acquire natural-abundance nitrogen-15 nuclear magnetic resonance spectra of biopolymers in solution.
Analyzed the relationship between molecular correlation times, spin-lattice relaxation times (T1), and nuclear Overhauser effects across multiple biomacromolecules, including lysozyme, protamines, pepsin, hemoglobin, vitamin B12, and tRNA.
Long correlation times characteristic of large biopolymers produced short spin-lattice relaxation times (T1), permitting rapid radiofrequency pulsing and accelerated spectral accumulation.
Unfavorable nuclear Overhauser effects partially offset the acquisition efficiency gained from shortened spin-lattice relaxation times.
Preliminary nitrogen-15 spectra were successfully obtained for diverse biopolymers, confirming detectable natural-abundance resonances in lysozyme, pepsin, hemoglobin, and tRNA.

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Implication

Spectroscopic study demonstrates the viability of natural-abundance nitrogen-15 NMR in large biopolymers, highlighting rapid signal accumulation despite unfavorable nuclear Overhauser effects.

Cite This Study

Gust et al. (1975) studied this question.

synapsesocial.com/papers/6aa17dbe556578a1cb44405d https://doi.org/10.1073/pnas.72.12.4696

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Implication

Spectroscopic study demonstrates the viability of natural-abundance nitrogen-15 NMR in large biopolymers, highlighting rapid signal accumulation despite unfavorable nuclear Overhauser effects.

Applications of natural-abundance nitrogen-15 nuclear magnetic resonance to large biochemically important molecules.

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Discussion

Implication

Cite This Study

Applications of natural-abundance nitrogen-15 nuclear magnetic resonance to large biochemically important molecules.

Key Points

Discussion

Implication

Cite This Study

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