Key result
Maximal short-term exercise increases TRAP-6 stimulated platelet reactivity by ~27% and elevates platelet-leukocyte conjugate formation.
Why the study?
Does maximal short-term exercise increase platelet activity and platelet-leukocyte conjugate formation in healthy non-smokers?
Does maximal short-term exercise increase platelet activity and platelet-leukocyte conjugate formation in healthy non-smokers?
Absolute Event Rate: 51.7% vs 40.6%
p-value: p=< 0.01
Maximal short-term exercise leads to a moderate, transient increase in platelet reactivity and platelet-leukocyte conjugate formation in healthy individuals.
May transiently heighten platelet reactivity after maximal exercise in healthy adults; leaves open relevance to thrombotic risk or patients with CVD.
Strenuous exercise may be partially responsible for cardio-vascular events. The aim was to investigate the platelet activity, reactivity and different platelet-leukocyte-conjugate formation following maximal short-term exercises. Fifteen healthy non-smokers underwent three isokinetic maximal tests on a SRM cycle ergometry system with durations of 15, 45 and 90 s. Blood samples were taken after a 30-min rest, immediately before and after exercise, and 15 min and 1 h after completion of exercise. Platelets were detected flow-cytometrically by CD41, and activated platelets by CD62P. In addition, stimulation of the platelets in vitro with 7.5 μM TRAP-6 was initiated. For testing platelet-leukocyte-conjugates, antibodies against CD45, CD14 and CD41 were used. After the exercise tests the percent of non-stimulated CD62P-positive platelets (%PC) was unchanged. In contrast, an increase in %PC (CD62P) TRAP-6 stimulated (15-s test: 37.2±10.3 to 46.2±12.3%, P < 0.05; 90-s test: 40.6±9.5 to 51.7±10.2%, P < 0.01) and in platelet-granulocyte, platelet-lymphocyte, and platelet-monocyte conjugate formation 15 min after exercise (45- and 90-s test; P < 0.05) were observed in comparison with the changes on the control day. The changes nearly reversed 1 h after exercise. Maximal short-term exercise only leads to a moderate increase of platelet reactivity and to an increase in the different platelet-leukocyte conjugates. The implications of the changes in platelet-leukocyte conjugate formation should be investigated in future studies.
No takes yet. Share an insight, caveat, or question.
Hilberg et al. (2003) studied Healthy non-smokers (n=15). Maximal short-term exercise vs. Rest / Control day was evaluated on TRAP-6 stimulated platelet reactivity (%PC) for the 90-s test (p=< 0.01). Maximal short-term exercise led to a moderate increase in TRAP-6 stimulated platelet reactivity (from 40.6% to 51.7% for the 90-s test; P<0.01) and platelet-leukocyte conjugate formation.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: