Key result
Inhibiting 60S ribosomal subunit joining silences 15-lipoxygenase mRNA translation in early erythroid precursor cells.
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Advances molecular models of erythroid translation control; leaves open in vivo roles and therapeutic targeting.
15-lipoxygenase (LOX) expression is translationally silenced in early erythroid precursor cells by a specific mRNA-protein complex formed between the differentiation control element in the 3' untranslated region (UTR) and hnRNPs K and E1. The 3'UTR regulatory complex prevents translation initiation by an unknown mechanism. We demonstrate that the 40S ribosomal subunit can be recruited and scan to the translation initiation codon even when the silencing complex is bound to the 3'UTR. However, the joining of the 60S ribosomal subunit at the AUG codon to form a translation competent 80S ribosome is inhibited, unless initiation is mediated by the IGR-IRES of the cricket paralysis virus. These findings identify the critical step at which LOX mRNA translation is controlled and reveal that 60S subunit joining can be specifically regulated.
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Ostareck et al. (2001) studied this question. Translational silencing of 15-lipoxygenase mRNA in early erythroid precursor cells occurs through the specific inhibition of 60S ribosomal subunit joining at the AUG codon.
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