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PCR cycle threshold (Ct) values are assay-specific signals that cannot serve as standalone surrogates for viable bacterial burden, necessitating their integration with traditional culture and antimicrobial susceptibility testing for accurate clinical decision-making. Novelty: ClaimNovelty.SYNTHESIS. Consensus alignment: ConsensusAlignment.NEUTRAL.

From Colonies to Copies: Integrating PCR, Culture, and AST in Bacterial Diagnostics

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