MicroRNA-29a attenuates angiotensin-II induced-left ventricular remodeling by inhibiting collagen, TGF-β and SMAD2/3 expression.

Figures

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  Figure 1.. Overexpression of miR-29a inhibits left ventricular remodeling by using ultrasound assessment. (A): Representative M-mode echocardiography images; and (B): echocardiography parameters were detected in mice among four groups (a. LVAW; d. the thickness of the LVAW at the end-diastolic; b. LVAW; s. the thickness of the LVAW at the end-systolic; c. LVPW; d. the thickness of the LVPW at the end-diastolic; d. LVPW; s. the thickness of the LVPW at the end-systolic; e. LVID; d. the left ventricular interior diameter at the end-diastolic; f. LVID; s. the LVID at the end-systolic; g. EF%: LV ejection fraction; h. FS%: LV fractional shortening; i. LV Vol; d. LV volume at the end-diastolic; and j. LV Vol; s. LV volume at the end-systolic.). Data are indicated as the means ± S.E. *P < 0.05, **P < 0.01, ***P < 0.001. EF: ejection fraction; FS: fractional shortening; LV: left ventricular; LVAW: left ventricular anterior wall; LVID: left ventricular inner dimension; LVPW: left ventricular posterior wall.

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  Figure 2.. Overexpression of miR-29a inhibits fibroblast proliferation. (A): Color images of the cardiac cross-sectional under the microscope (200 ×) after Masson staining to observe the ventricular wall thickness; (B): color images of the cardiac cross-sectional after Sirius red Staining, in which four arrows show the expression of the LV myocardial collagen, and the collagen and non-collagen components is red and orange stained respectively; and (C): scatter plots of the relevant parameters detected after staining (a: the area of cardiac myocyte cross-section; and b: the LV collagen volume.). Scale bar is 1000 µm in Figure A & B. Data are indicated as means ± S.E. *P < 0.05, **P < 0.01. LV: left ventricular.

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  Figure 3.. Inhibition of miR-29a on cardiac collagen. (A): The image showing the expression of myocardial tissue collagen I and III under the fluorescence microscope (400 ×); and (B): the levels of collagen I and III. Data are indicated as the means ± S.E. *P < 0.05, miR-29a-mimic group vs. model group; #P < 0.05, miR-29a-KD group vs. miR-29a-mimic group. miR-29a-KD: miR-29a-knockdown.

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  Figure 4.. Inhibition of miR-29a on the expression of Collagen I, Collagen III, TGF-β and SMAD2/3. The expression of Collagen I, Collagen III, TGF-β and p-SMAD2/3 were detected using Western blot and the mimic group was low as compared with the model group (A & B), while the knockdown group was no obvious difference (C & D). Data are indicated as means ± S.E. **P < 0.01. TGF-β: transforming growth factor-β.