What question did this study set out to answer?

To determine the intracellular trafficking pathway and sequence of membrane targeting signals required for Ras proteins to reach the plasma membrane.

July 1, 1999CellOpen Access

Endomembrane Trafficking of Ras

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Authors

ECEdwin ChoyHarvard University VCVi K. ChiuAngeles Clinic and Research Institute JSJoseph SillettiNew York University

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Implication

Cellular study demonstrates that Ras proteins traffic through the Golgi before reaching the plasma membrane, revealing a multi-step endomembrane transport mechanism guided by CAAX motifs.

Key Points

To determine the intracellular trafficking pathway and sequence of membrane targeting signals required for Ras proteins to reach the plasma membrane.
Monitored live-cell subcellular localization and vesicular movement of green fluorescent protein (GFP)-tagged Ras isoforms (Nras, Hras, and Kras4B).
Interrupted secretory trafficking using prolonged brefeldin A (BFA) treatment and evaluated the functional roles of the CAAX motif and secondary targeting signals.
GFP-Nras and GFP-Hras localized transiently to the Golgi and motile peri-Golgi vesicles before plasma membrane expression, whereas GFP-Kras4B showed minimal Golgi and no vesicular accumulation.
Prolonged brefeldin A treatment completely blocked Nras delivery to the plasma membrane.
The CAAX motif was necessary and sufficient for initial targeting to the endomembrane and subsequent methylation, but a secondary targeting signal was required for final plasma membrane export.

Cite This Study

Choy et al. (1999) studied this question.

synapsesocial.com/papers/6aa244b41f0a798fe527ca2f https://doi.org/10.1016/s0092-8674(00)80607-8

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Implication

Cellular study demonstrates that Ras proteins traffic through the Golgi before reaching the plasma membrane, revealing a multi-step endomembrane transport mechanism guided by CAAX motifs.

Endomembrane Trafficking of Ras

Discussion

Member takes

Implication

Key Points

Cite This Study

Endomembrane Trafficking of Ras

Discussion

Member takes

Implication

Key Points

Cite This Study

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