Key result
Compounds that increase intracellular cAMP induced dose- and time-dependent secretion of tPA and vWF from cultured human endothelial cells without increasing intracellular calcium.
Population
Cultured human umbilical vein endothelial cells (HUVEC)
Design
Preclinical
Authors
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Does not support clinical use of cAMP modulators; leaves open in vivo relevance for thrombosis risk.
An increase in intracellular cAMP induces regulated secretion of tPA and vWF from endothelial cells via a calcium-independent mechanism.
Hegeman et al. (1998) studied this question. cAMP-elevating compounds was evaluated on Secretion of tissue-type plasminogen activator (tPA) and von Willebrand factor (vWF). Compounds that increase intracellular cAMP induced dose- and time-dependent secretion of tPA and vWF from cultured human endothelial cells without increasing intracellular calcium.
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