Anti-thrombocytopenic and Haemostatic Effect of an Unani Formulation on Busulfan-induced Thrombocytopenia in Rats

Background: The Unani medical system uses single medications or formulations, which are combinations of multiple drugs, to manage a variety of ailments. The Unani physician used these formulations in their clinics. A Polyherbal Unani Formulation (PUF) was selected to explore its Anti-thrombocytopenic activity. This formulation is used in fever, and based on its ingredients, the Anti-thrombocytopenic activity was explored. Aim: The research aimed to examine the anti-thrombocytopenic and hemostatic effect of a compound Unani herbal formulation in the busulfan-induced male Sprague Dawley (SD) rats. Methods: Busulfan-induced thrombocytopenia method was used in this study. Preventive and curative regimens were tested, and platelet counts, bleeding time, clotting time, Haematological and biochemical blood tests were carried out. The histopathology was also done at the end of the experiments. The decoction of the PUF was used in the male SD rats for this study. Results: The PUF did not show anti-thrombocytopenic activity in the preventive regimen, but in the curative regimen, it exhibited increased platelet count, much better in animals than busulfan control animals and showed mild anti-thrombocytopenic activity. It has also shown hepatoprotective and nephroprotective potential in the preventive and curative regimen. Conclusion: PUF exhibited mild anti-thrombocytopenic activity in the curative study and hepatoprotective and nephroprotective potential in this study. Major Findings: Present study demonstrated the mild anti-thrombocytopenic activity in curative regimen, and hepatoprotective as well as nephroprotective potential of a traditional Unani formulation in preventive and curative regimen.