A Simplified and Robust LC–MS/MS Method for Simultaneous Quantification of Six Steroid Hormones Associated With Polyendocrine Metabolic Ovarian Syndrome
Validation study demonstrates rapid quantification of six steroid hormones, indicating feasibility for routine polyendocrine metabolic ovarian syndrome evaluation.
Key Points
To establish and validate a simplified, robust liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of six steroid hormones linked to polyendocrine metabolic ovarian syndrome.
Coupled liquid-liquid extraction with protein precipitation for biological sample preparation.
Conducted chromatographic separation within a 6-minute run time using LC-MS/MS.
Evaluated assay performance according to international guidelines for linearity, sensitivity, precision, accuracy, matrix effects, selectivity, and specificity.
All six steroid hormones demonstrated linearity with correlation coefficients of R = 0.9959 to 0.9995, intra- and inter-day coefficients of variation ≤ 5.6%, and spike recovery rates between 85.2% and 114.9%.
The method achieved an expanded linear dynamic range for dehydroepiandrosterone sulfate spanning 20 to 20,000 ng/mL.