Key result
The RT-LAMP assay detected foot-and-mouth disease virus C with a limit of 3.25 × 10^-1 ng/mL, making it 10 times more sensitive than conventional RT-PCR without cross-reactivity to other serotypes.
Absolute Event Rate: 0.325% vs 3.25%
RT-LAMP provides a rapid, highly sensitive, and specific method for the clinical diagnosis and surveillance of foot-and-mouth disease virus serotype C.
May aid rapid veterinary FMDV detection; leaves open field validation before clinical adoption.
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.
No takes yet. Share an insight, caveat, or question.
Ding et al. (2014) studied Foot-and-mouth disease virus serotype C (n=41). RT-LAMP assay vs. Conventional RT-PCR was evaluated on Detection limit (sensitivity). The RT-LAMP assay detected foot-and-mouth disease virus C with a limit of 3.25 × 10^-1 ng/mL, making it 10 times more sensitive than conventional RT-PCR without cross-reactivity to other serotypes.