Key result
Hypoxia significantly decreased mitochondrial cristae volume and increased matrix volume, whereas positive inotropic drugs caused no significant ultrastructural changes in isolated rabbit hearts.
Why the study?
Does exposure to hypoxia or positive inotropic drugs alter myocardial ultrastructure in isolated rabbit hearts?
Does exposure to hypoxia or positive inotropic drugs alter myocardial ultrastructure in isolated rabbit hearts?
Hypoxia, but not positive inotropic drugs at the tested concentrations, alters myocardial ultrastructure in the isolated rabbit heart model.
Hypoxia-induced mitochondrial shifts in this model warrant no change in inotrope use; leaves open clinical translation.
The isolated spontaneously beating heart was used for comparing the effects of hypoxia and positive inotropic drugs on myocardial ultrastructure. Hypoxia gives a significant decrease in the volume fractions of mitochondrial cristae relative to the total mitochondrial volume (Vvmcristae) and a significant increase in the volume fraction of mitochondrial matrix relative to the total mitochondrial volume (Vvmmatrix), but changes in volume fractions of mitochondria (Vvmitochondria) and myofibrils (Vvmyofibrils) were absent. Significant changes in ultrastructure in hearts treated with dopamine (0.6 microM), dobutamine (90 nM), G-strofanthin (0.25 microM), xamoterol (32 nM) and isoprenaline (0.15 microM or 15 nM) were absent. Furthermore, myocardial effects in the isolated rabbit heart without exposure to any treatment showed a significantly decrease in oxygen consumption after 90 min. and a significant decrease in frequency of contractions after 120 min. perfusion time, but no change in contractility was seen. We conclude that this experimental model is useful in studies of positive inotropic drugs.
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Jensen et al. (1992) studied Isolated rabbit heart model. Hypoxia and positive inotropic drugs (dopamine, dobutamine, G-strofanthin, xamoterol, isoprenaline) vs. No treatment was evaluated on Myocardial ultrastructure (volume fractions of mitochondrial cristae, matrix, mitochondria, and myofibrils). Hypoxia significantly decreased mitochondrial cristae volume and increased matrix volume, whereas positive inotropic drugs caused no significant ultrastructural changes in isolated rabbit hearts.
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