Key result
RT-PCR detects foot and mouth disease virus in ~58% of symptomatic cattle samples.
Why the study?
Following an outbreak of foot and mouth disease virus in Indonesia, the study aimed to detect the causative agent in cattle with vesicle lesions in the mouth and hooves.
Cross-Sectional (n=12)
RT-PCR is an effective method for the rapid and accurate diagnosis of Foot and Mouth Disease virus in symptomatic cattle.
Supports RT-PCR for FMDV detection in symptomatic cattle; leaves open validation in larger prospective cohorts.
Foot and mouth disease (FMD) virus was reported as an outbreak in Indonesia in April 2022 and belonged to serotype O/ME-SA/Ind-2001e is spread in the country. This study aimed to detect the causative agent based on clinical symptoms in cattle that have the vesicle in the mouth and hooves. A total of 25 samples were collected during August 2022 from Lamongan and Surabaya, Indonesia. FMD was identified in 58% (7/12) using reverse transcription polymerase chain reaction (RT-PCR), respectively. The samples were performed using universal primers with 328 bp length for primary diagnosis of FMD. These findings indicate that the spread of FMD viruses is highly contagious, so rapid and accurate diagnosis is needed as an effort to control and monitor FMD viruses.
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Dinana et al. (2023) conducted a cross-sectional in Foot and Mouth Disease (n=12). RT-PCR was evaluated on Detection rate of FMD-positive samples. Foot and mouth disease virus was successfully detected in 58% (7/12) of symptomatic cattle samples from Lamongan and Surabaya, Indonesia using the RT-PCR method.
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