Unique Nucleotide Polymorphism of African Swine Fever Virus Circulating in East Asia and Central Russia

Figure 1. Epidemic situation on ASF in the Russian Federation from 2019 to 2022. The isolates covered by this study are indicated in large print.

Figure 2. The ratio of absorbance at A260/A230 (low molecular weight purification rate, reference ≥ 2.0) (A), number of reads (B), A260/A280 (protein purification rate, reference ≥ 1.8) wavelengths for gDNA samples (C), number of ASFV specific reads (D), and mean coverage (E).

Figure 3. Percent of identity between the studied isolates and ASFV reference strains characterized in geographically separated areas of Eurasia.

Figure 4. Phylogram showing relatedness between the twenty-one isolates from this study and the most well-known ASFV reference strains from Genbank. Note: the isolates covered by this research are labeled with •.

Figure 5. Maximum likelihood phylogenetic tree indicating the relationship of ASFV genotype II sequences, using selected ORFs MGF 110-1L (A), MGF 505-9R (B), NP419L, (C) and I267L (D). Note: the isolates covered in this study are labeled with a black dot; the original (root) and Genetic variant (I) are marked with blue while Genetic variant (II) is green.

Figure 6. (A) Nucleotide sequence alignment of the intergenic region B602L/B385R of ASFV genotype II isolates, showing the formation of two variants. (B) Maximum likelihood phylogenetic tree of showing the relation between ASFV isolates based on the analysis of MGF 360-10L. Variant II is indicated in green; other isolates belong to cluster Asia (blue). Isolates from this study are labeled with a black dot.

Figure 7. Alignment of IGR I73R/I329L sequences after genome assembly (A) and after Sanger sequencing (B). Note: The blue color indicates the IGR-I variant; the green color indicates IGR-II; the isolates covered in this study are labeled black background color.

Figure 8. The molecular and epidemiological pattern of ASFV genotype II circulation in Europe and Asia, based on whole genome analysis of isolates.