Proteinadsorption auf alkylsubstituierten Oberflächen

When confronted with the chromatographic separation of proteins one often has to cope with a large number of parameters which have either been set by the producer or oneself and which in many cases are only poorly understood. Therefore in this paper some of the fundamentals involved in the mechanism of protein adsorption on alkyl‐substituted gel surfaces will be treated. Although a large number of papers have been published on protein adsorption there is no general theory on the adsorption mechanism. The possibility of synthesizing surfaces for adsorption in a controlled way on hydrophilic gels has become feasible after the introduction of affinity chromatography. Since this technique has allowed the elucidation of a number of general mechanistic principles involved in the protein adsorption process and the corresponding sorption kinetics these results will be primarily discussed here.