What question did this study set out to answer?

To develop and evaluate a two-compartment in vitro test model to quantify bacterial penetration into dentinal tubules.

January 1, 2000International Endodontic Journal

Penetration of bacteria in bovine root dentine in vitro

View Full Paper

Ask AI

Bookmark

Authors

LPL. B. PetersAcademic Center for Dentistry Amsterdam PWP. R. WesselinkAcademic Center for Dentistry Amsterdam WMW.R. MoorerAcademic Center for Dentistry Amsterdam

Discussion

Member takes

Implication

In vitro study demonstrates deeper bacterial penetration in bovine root dentine after smear layer removal, highlighting challenges for endodontic disinfection.

Key Points

To develop and evaluate a two-compartment in vitro test model to quantify bacterial penetration into dentinal tubules.
Mounted 1.5–3.1 mm thick bovine root dentine specimens (N=72, cementum removed) between an inoculated compartment and a sterile broth compartment for 6 weeks.
Treated dentine surfaces to preserve or remove the smear layer using EDTA, saline, or saline combined with sodium hypochlorite before exposure to Enterococcus faecalis or Actinomyces israelii.
Quantified bacterial penetration depth using light microscopy histology alongside a recovering grinding technique that cultured infected dentine dust.
E. faecalis penetrated dentine significantly deeper than A. israelii (P < 0.001).
Smear layer removal with EDTA led to significantly deeper E. faecalis penetration compared with saline alone (P < 0.01) or saline with sodium hypochlorite (P < 0.01).
Bacteria were detected in 89% of cultured dentine dust samples and 80% of microscopic evaluations, with complete penetration into the broth compartment occurring in 2 of 72 specimens.

Cite This Study

Peters et al. (2000) studied this question.

synapsesocial.com/papers/6aa2d4d00fc92d3596e570d1 https://doi.org/10.1046/j.1365-2591.2000.00268.x

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates deeper bacterial penetration in bovine root dentine after smear layer removal, highlighting challenges for endodontic disinfection.

Penetration of bacteria in bovine root dentine in vitro

Discussion

Member takes

Implication

Key Points

Cite This Study

Penetration of bacteria in bovine root dentine in vitro

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider