What question did this study set out to answer?

Validate an integrated enzymatic-gravimetric and liquid chromatography method to quantify total dietary fiber in food according to the CODEX Alimentarius definition.

January 1, 2010Journal of AOAC International

Determination of Total Dietary Fiber (CODEX Definition) by Enzymatic-Gravimetric Method and Liquid Chromatography: Collaborative Study

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Authors

BMBarry V. McClearyTotal (France)JDJonathan W DeVriesSouthwestern Medical Center JRJeanne I. RaderCenter for Food Safety and Applied Nutrition

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Implication

Collaborative interlaboratory study demonstrates precise total dietary fiber quantitation in diverse food samples, indicating suitability for official regulatory adoption.

Key Points

Validate an integrated enzymatic-gravimetric and liquid chromatography method to quantify total dietary fiber in food according to the CODEX Alimentarius definition.
Conducted an AOAC collaborative study involving 18 laboratories (16 returned valid assay data) across 16 test portions (eight blind duplicates).
Employed extended enzymatic digestion at 37°C simulating human digestion, isolating high-molecular-weight dietary fiber gravimetrically and low-molecular-weight soluble dietary fiber via liquid chromatography.
Total dietary fiber content across the eight duplicate food sample pairs ranged from 11.57% to 47.83%.
Repeatability standard deviations (Sr) ranged from 0.41 to 1.43, and reproducibility standard deviations (SR) ranged from 1.18 to 5.44.

Cite This Study

McCleary et al. (2010) studied this question.

synapsesocial.com/papers/6aa2daf76401f02f7be572b0 https://doi.org/10.1093/jaoac/93.1.221

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Implication

Collaborative interlaboratory study demonstrates precise total dietary fiber quantitation in diverse food samples, indicating suitability for official regulatory adoption.

Determination of Total Dietary Fiber (CODEX Definition) by Enzymatic-Gravimetric Method and Liquid Chromatography: Collaborative Study

Discussion

Member takes

Implication

Key Points

Cite This Study

Determination of Total Dietary Fiber (CODEX Definition) by Enzymatic-Gravimetric Method and Liquid Chromatography: Collaborative Study

Discussion

Member takes

Implication

Key Points

Cite This Study

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