The oxidation of exogenous reduced nicotinamide-adenine dinucleotide by plant mitochondria

Chloroform did not extract colour from 'bili- mucin' and t.l.c. in pentyl acetate-acetic acidwater (5: 5: 2, by vol.) gave a continuous streak and no discrete spots corresponding to bile salts.The ethanolic liquors from the original precipita- tion in one case were evaporated in N2 at reduced pressure in the dark at about 55°C.From a Sephadex G-100 column, the coloured compounds in the residue were eluted at column volume.'Bilimucin' appears to be a macromolecular complex in which bilirubin might be linked to mucopolysaccharide.