Biosynthesis and antimicrobial activity of copper oxide nanoparticles

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The development of simple, economical, and effective technologies to produce nanoparticles is a global priority. Microorganism-based biosynthesis methods developed as an environmentally conscious, pristine, and feasible alternative to conventional physical and chemical approaches. This research outlines the synthesis of copper oxide nanoparticles utilizing bacterial filtrated supernatant. The investigation focused on variables such as pH, copper concentration, and temperature. Optimal conditions for nanoparticle formation were identified at a neutral pH, 4 mM copper concentration, and 35°C. Characterization of the CuO NPs involved TEM, UV-Vis spectroscopy, XRD, and FTIR to examine their pure form, revealing a maximum particle size of 36.22 nm with spherical morphology. Evaluating the antimicrobial efficacy on pathogenic bacteria (Staphylococcus aureus and Pseudomonas aeruginosa) and plant pathogenic fungi (Rhizoctonia solani and Fusariumsolani) through the well diffusion method affirmed the probable outcome of CuO-NPs as an efficient antimicrobial agent, supporting and validating prior assertions