Costal tissues were analyzed biochemically in respect to a profile of sulfated mucopolysaccharides from normal and rachitic calves which provided large samples of anatomically identifiable regions for biochemical analysis. Histologic distribution of these compounds was obtained through the use of x‐ray elemental analysis for sulfur. Distinctive differences between polysaccharide content of normal and rachitic tissues were found and calcification in vitro of the rachitic cartilage was demonstrated.
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David S. Howell (1965) studied this question.
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