Key result
Coronary and tracheal detergent perfusion completely decellularizes rat heart-lung blocks while preserving wall structure.
Why the study?
Bioengineered transplantable heart-lung scaffolds could be potentially lifesaving in congenital and acquired cardiothoracic disorders, including terminal heart-lung disease.
A novel combined perfusion decellularization heart-lung model was successfully developed in rats, representing a first step toward bioengineered transplantable heart-lung scaffolds.
Answers are grounded in this paper and cite their sources.
Hypothesis-generating for bioengineered thoracic organs; leaves open scalability, recellularization, and in vivo function in larger models.
BACKGROUND: Bioengineered transplantable heart-lung scaffolds could be potentially lifesaving in a large number of congenital and acquired cardiothoracic disorders including terminal heart-lung disease. METHODS: We decellularized heart-lung organ-blocks from rats (n = 10) by coronary and tracheal perfusion with ionic detergents in a modified Langendorff circuit. RESULTS: In the present project, we were able to achieve complete decellularization of the heart-lung organ-block. Decellularized heart-lung organ-blocks lacked intracellular components but maintained structure of the cellular walls with collagen and elastic fibers. CONCLUSIONS: We present a novel model of combined perfusion and decellularization of heart-lung organ-blocks. This model is the first step on the pathway to creating bioengineered transplantable heart-lung scaffolds. We believe that further development of this technology could provide a life-saving conduit, significantly reducing the risks of heart-lung failure surgery and improving postoperative quality of life.
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Zubarevich et al. (2022) studied terminal heart-lung disease (n=10). Coronary and tracheal perfusion with ionic detergents was evaluated on Complete decellularization of the heart-lung organ-block. Coronary and tracheal perfusion with ionic detergents achieved complete decellularization of heart-lung organ-blocks in rats, maintaining cellular wall structure with collagen and elastic fibers.
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