Key result
Dual-wavelength ratiometric fluorescence suppresses motion artifacts in MAP recordings from isolated rabbit hearts.
Dual-wavelength ratiometric fluorescence measurement is an effective method for eliminating motion artifacts during simultaneous optical mapping of action potentials and electrograms in isolated hearts.
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Improves optical mapping reliability in isolated hearts; leaves open translation to in vivo or clinical electrophysiology.
Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.
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Kolářová et al. (2010) studied Isolated heart electrophysiology (n=6). Dual-wavelength ratiometric fluorescence emission measurement vs. Single-wavelength measurement was evaluated on Reduction of motion artifacts in monophasic action potential recordings. Dual-wavelength ratiometric fluorescence emission measurement effectively suppressed motion artifacts in monophasic action potential recordings from isolated rabbit hearts.
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