Key result
In budding yeast, the RNA helicase Ded1 and its C-terminal region regulate translation and stress granule formation during both the initial response to oxidative stress and the subsequent recovery phase.
Why the study?
Ded1 regulates translation and stress granule dynamics during cellular stress, but its precise role in stress responses required better understanding.
The RNA helicase Ded1 controls multiple aspects of translation and RNP dynamics during both initial cellular stress responses and recovery.
Yeast model implicates RNA helicase in stress recovery; leaves open extension to mammalian cardiovascular stress responses.
Ded1 is a conserved RNA helicase that promotes translation initiation in steady-state conditions. Ded1 has also been shown to regulate translation during cellular stress and affect the dynamics of stress granules (SGs), accumulations of RNA and protein linked to translation repression. To better understand its role in stress responses, we examined Ded1 function in two different models: DED1 overexpression and oxidative stress. DED1 overexpression inhibits growth and promotes the formation of SGs. A ded1 mutant lacking the low-complexity C-terminal region ( ded1-ΔCT ), which mediates Ded1 oligomerization and interaction with the translation factor eIF4G, suppressed these phenotypes, consistent with other stresses. During oxidative stress, a ded1-ΔCT mutant was defective in growth and in SG formation compared to wild-type cells, although SGs were increased rather than decreased in these conditions. Unlike stress induced by direct TOR inhibition, the phenotypes in both models were only partially dependent on eIF4G interaction, suggesting an additional contribution from Ded1 oligomerization. Furthermore, examination of the growth defects and translational changes during oxidative stress suggested that Ded1 plays a role during recovery from stress. Integrating these disparate results, we propose that Ded1 controls multiple aspects of translation and RNP dynamics in both initial stress responses and during recovery.
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Aryanpur et al. (2021) studied Cellular stress. DED1 overexpression and oxidative stress (hydrogen peroxide) vs. Wild-type or untreated cells was evaluated on Cell growth, translation repression, and stress granule formation. In budding yeast, the RNA helicase Ded1 and its C-terminal region regulate translation and stress granule formation during both the initial response to oxidative stress and the subsequent recovery phase.
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