SummaryThrough inbreeding we obtained diploid drones in 5 colonies of Melipona quadrifasciata in the ratio of 1 male to 1 female (workers + queens). These colonies continued to produce queens, so we conclude that a new locus from the X series was present, with 3 or more alleles; x° (° = ovary). Individuals hemizygous for x° (x°1, x°2,…. x°n) and homozygous (x°1/x°1,…. x°n/x°n) would be haploid and diploid males, respectively, and heterozygous individuals would be females.
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C. A. Camargo (1979) studied this question.
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