What question did this study set out to answer?

To determine whether GTP-binding regulatory proteins participate in protein transport between successive compartments of the Golgi stack.

December 1, 1987CellOpen Access

Involvement of GTP-binding “G” proteins in transport through the Golgi stack

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Authors

PMPaul MelançonUniversity of Alberta BGBenjamin S. GlickUniversity of Chicago VMVivek MalhotraInstitució Catalana de Recerca i Estudis Avançats

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Implication

In vitro experimental study demonstrates that non-hydrolyzable GTP analogs arrest intra-Golgi transport, indicating G protein regulation of vesicle fusion.

Key Points

To determine whether GTP-binding regulatory proteins participate in protein transport between successive compartments of the Golgi stack.
Monitored inter-compartmental protein transport in a cell-free Golgi system treated with GTP-gamma-S and aluminum-potentiated fluoride.
Performed preincubation assays to evaluate donor versus acceptor membrane vulnerability and used electron microscopy to examine Golgi cisternae ultrastructure.
GTP-gamma-S and fluoride plus aluminum irreversibly inhibited intra-Golgi transport via a cytosolic factor that selectively acts on acceptor membranes between vesicle attachment and fusion.
Electron microscopy demonstrated a 5-fold accumulation of non-clathrin-coated buds and vesicles associated with Golgi cisternae during GTP-gamma-S inhibition.

Cite This Study

Melançon et al. (1987) studied this question.

synapsesocial.com/papers/6aa3ddf9c715cee61e21dec3 https://doi.org/10.1016/0092-8674(87)90591-5

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Implication

In vitro experimental study demonstrates that non-hydrolyzable GTP analogs arrest intra-Golgi transport, indicating G protein regulation of vesicle fusion.

Involvement of GTP-binding “G” proteins in transport through the Golgi stack

Discussion

Member takes

Implication

Key Points

Cite This Study

Involvement of GTP-binding “G” proteins in transport through the Golgi stack

Discussion

Member takes

Implication

Key Points

Cite This Study

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