What question did this study set out to answer?

To construct a physical cleavage map and locate denaturation regions on supercoiled human papillomavirus DNA isolated from plantar warts.

Human papillomavirus DNA: physical map.

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Laboratory study demonstrates restriction endonuclease cleavage patterns of HPV DNA, highlighting the physical organization of the viral genome.

To construct a physical cleavage map and locate denaturation regions on supercoiled human papillomavirus DNA isolated from plantar warts.
Purified supercoiled form I HPV DNA from plantar warts and cleaved it using restriction endonucleases EcoRI, Hind (unfractionated Hind, HindII, HindIII), and HpaI.
Analyzed DNA cleavage fragments and circular complexes treated with bacteriophage T4 gene 32 protein using polyacrylamide gradient slab gel electrophoresis and electron microscopy.
EcoRI cleaved HPV DNA into two fragments representing 87% and 13% of the genome, establishing an EcoRI site as the zero reference point on the physical map.
Hind endonucleases digested the genome into six fragments ranging from 33.5% to 1.2% of total genome length via three HindII sites (two shared with HpaI) and three HindIII sites.
Reaction with bacteriophage T4 gene 32 protein followed by restriction digestion identified and mapped two readily denatured regions on the circular viral genome.

Fãvre et al. (1975) studied this question.

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View Full Paper

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Authors

Laboratory study demonstrates restriction endonuclease cleavage patterns of HPV DNA, highlighting the physical organization of the viral genome.

To construct a physical cleavage map and locate denaturation regions on supercoiled human papillomavirus DNA isolated from plantar warts.
Purified supercoiled form I HPV DNA from plantar warts and cleaved it using restriction endonucleases EcoRI, Hind (unfractionated Hind, HindII, HindIII), and HpaI.
Analyzed DNA cleavage fragments and circular complexes treated with bacteriophage T4 gene 32 protein using polyacrylamide gradient slab gel electrophoresis and electron microscopy.
EcoRI cleaved HPV DNA into two fragments representing 87% and 13% of the genome, establishing an EcoRI site as the zero reference point on the physical map.
Hind endonucleases digested the genome into six fragments ranging from 33.5% to 1.2% of total genome length via three HindII sites (two shared with HpaI) and three HindIII sites.
Reaction with bacteriophage T4 gene 32 protein followed by restriction digestion identified and mapped two readily denatured regions on the circular viral genome.

Fãvre et al. (1975) studied this question.

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