Serum Disease-Specific IgG Fc Glycosylation as Potential Biomarkers for Nonproliferative and Proliferative Diabetic Retinopathy Using Mass Spectrometry

@PANI) were used to enrich IgG N-glycopeptides from tryptic digestion. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI ToF MS) was used to detect the IgG N-glycopeptides. Nine DSIgG N-glycopeptide ratios were significantly different among NDR, NPDR, and PDR groups. There are six glycopeptide ratios available to classify mild, moderate, and severe NPDR. Moreover, four glycopeptide ratios could identify patients with or without diabetic macular edema (DME). The prediction model exhibited good discriminatory performance in distinguishing patients with DR or NDR (AUC = 0.8347), NPDR or PDR (AUC = 0.7002), mild/moderate or severe NPDR (AUC = 0.8059), and with or without DME (AUC = 0.7846). DSIgG Fc N-glycosylation ratios were closely associated with different stages of DR and may be used as potential biomarkers for the early diagnosis of NDR, NPDR, and PDR.